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85082303

FS6-14.13 Ag2.1 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81
Pricing and availability is not currently available.

biological source

Mouse x Mouse T cell Hybridoma

Quality Level

form

liquid

packaging

tube of 5 μg 85082303-DNA-5UG
pkg of vial of cells 85082303-1VL

ImmunoDon

B6DF mouse T cells

Myeloma

Azaguanine resistant BW5147 AKR mouse tumour cells

Reactivity

T cell hybridoma

technique(s)

cell culture | mammalian: suitable

Cell Line Description

This cell line is a stable T cell hybridoma which, when stimulated with concanavalin A, produces IL-2. The cell surface markers include Thy1.2 and H-2b products.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Culture Medium

RPMI 1640 + 2 mM glutamine + 1 mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS).

Subculture Routine

Maintain cultures between 2-6 x 100,000 cells/ml; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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L Harwell et al.
The Journal of experimental medicine, 152(4), 893-904 (1980-10-01)
The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells

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