80701

Crystallization Kit for DNA

• Synonym(s): DNA Crystallization Kit
• UNSPSC Code: 12171500

Properties

• storage temp.: 2-8°C

Description

General description

The Crystallization Kit for DNA is an empirical screening method for the direct determination of suitable crystallization conditions for nucleic acids.

The kit is developed according to the described method of Nowakowski et al.[1], Baeyens, Jancarik and Holbrook[2] These ready-made solutions represent a screen of conditions, from which many were observed to produce crystals from DNA oligomers and transfer RNA. This screen provides high efficiency in crystallization of RNA oligomers. Thus it provides a completion of our crystallization kit for DNA.

The Crystallization Kit for DNA consists of 48 different formulations covering a range of different buffers (pH 6.0-8.0), different types of precipitants (esp. PEG 400 and MPD), and inorganic salts including magnesium salts. All solutions are sterile filtered using 0.22 micron filters. The kit contains 10 ml of each formulation, but all solutions are available separately as 100 ml bottles. Larger quantities are available on request.

Features and Benefits

• Comprehensive, efficient screening
• Extremely pure, sterile reagents
• Prefabricated reagents and observation sheets
• Low incidence of undesirable solubility effects

Other Notes

Every component can be ordered separately in pack size of 100 mL, but inquiries for different sizes are also welcome.

The Kit contains 48 components with 10 mL each.

Kit Components Only

• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 5 mM − Cobalt(III)-hexamine 2.5 mM solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Sodium citrate 200 mM − Isopropanol 5% solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 15 mM − Spermine 10.0 mM − Lithium sulfate 2.0 M solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 1.5 mM − Ammonium sulfate 3.0 solution
• Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Sodium chloride 2.5 M solution
• Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Cobalt(III)-hexamine 1.0 mM − Lithium chloride 2.0 M solution
• TRIS pH 7.5; 0.05 M − Magnesium chloride 200 mM − Sodium chloride 2.5 M solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 200 mM − Potassium chloride 2.5 M solution
Cacodylate pH 6.5; 0.05 M − Spermine 1.0 mM − Cobalt(III)-hexamine 2.0 mM − Calcium chloride 30 mM − Lithium chloride 2.0 M solution

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Kit Components Also Available Separately

• HEPES pH 7.5; 0.05 M − Magnesium chloride 80 mM − Spermine 2.5 mM solution
• Cacodylate pH 6.0; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Copper sulfate 1 mM − Isopropanol 9% solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 18 mM − Spermine 0.9 mM − Cobalt(III)-hexamine 1.8 mM − Isopropanol 9% solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Isopropanol 9% solution
• Cacodylate pH 7.0; 0.05 M − Magnesium chloride 18 mM − Spermine 2.25 mM − Cobalt(III)-hexamine 0.9 mM − 2-Methyl-2,4-pentanediol 4.5% solution
• Cacodylate pH 6.5; 0.05 M − Magnesium chloride 36mM − Spermine 2.25 mM − PEG 400 5% solution
• Sodium succinate pH 5.5; 0.05 M − Magnesium chloride 10 mM − Cobalt(III)-hexamine 2.0 mM − Isopropanol 10% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Ethanol 15% solution
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 15% solution
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 5 mM − Spermidine 1.0 mM − tert-Butanol 10% solution
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 30 mM − Spermine 2.5 mM − PEG 400 5% solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 100 mM − Cobalt(III)-hexamine 2.0 mM − Isopropanol 5% solution
TRIS pH 8.0; 0.05 M − Magnesium chloride 10 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 20% solution
HEPES pH 7.5; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − PEG 8000 5% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 2.5 mM − PEG 4000 5% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 10 mM − Spermine 2.5 mM − Calcium chloride 5 mM − Isopropanol 10% solution
Cacodylate pH 7.0; 0.05 M − Magnesium chloride 9 mM − Spermine 2.25 mM − Cobalt(III)-hexamine 1.8 mM − Spermidine 0.9 mM − PEG 400 5% solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 2.5 mM − Copper sulfate 1 mM − Isopropanol 10% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Calcium chloride 2 mM − 1,5 Hexanediol 10% solution
HEPES pH 7.5; 0.05 M − Magnesium chloride 15 mM − Spermidine 1.0 mM − Dioxan 10% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 15 mM − Spermine 3.0 mM − PEG 400 10% solution
Cacodylate pH 6.5; 0.05 M − Spermine 2.5 mM − Calcium chloride 18 mM − 2-Propanol 9% solution
Cacodylate pH 6.5; 0.05 M − Spermine 2.0 mM − Cobalt(III)-hexamine 1.0 mM − Calcium chloride 80 mM solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 30 mM − Spermine 1.0 mM − Lithium sulfate 1.3 M solution
Cacodylate pH 6.0; 0.05 M − Calcium acetate 200 mM − Isopropanol 5% solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 100 mM − Cobalt(III)-hexamine 1.0 mM − Ethanol 10% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 10 mM − Spermidine 2.5 mM − Sodium chloride 2.5 M solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 20 mM − Spermine 1.0 mM − Ammonium sulfate 2.0 M solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 15 mM − Spermidine 5.0 mM − Cobalt(III)-hexamine 1.0 mM − Sodium chloride 2.0 M solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 200 mM − Sodium chloride 100 mM − PEG 1000 20% solution
TRIS pH 7.5; 0.05 M − Magnesium chloride 50 mM − Sodium tartrate 1.0 M solution
TRIS pH 8.0; 0.05 M – Magnesium chloride 200 mM – Ethanol 15% solution
Cacodylate pH 6.0; 0.05 M − Magnesium chloride 15 mM − Spermidine 5.0 mM − Lithium sulfate 2.0 M solution
Cacodylate pH 6.0; 0.05 M − Magnesium acetate 20 mM − Spermine 0.5 mM − Sodium chloride 100 mM − 2-Methyl-2,4-pentanediol 25% solution
Sodium succinate 0.05 M − Magnesium chloride 20 mM − Spermine 0.5 mM − Ammonium sulfate 3.0 solution
Cacodylate pH 6.5; 0.05 M − Cobalt(III)-hexamine 5 mM − Potassium chloride 2.5 M solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 50 mM − Cobalt(III)-hexamine 2.0 mM − Lithium sulfate 1.5 M solution
Cacodylate pH 6.5; 0.05 M − Magnesium chloride 10 mM − Spermine 50 mM solution

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