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Key Documents

77857

Sigma-Aldrich

Ketoreductase

recombinant, expressed in E. coli, ≥0.2 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

powder

specific activity

≥0.2 U/mg

storage temp.

2-8°C

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Application

Ketoreductases are used to study the stereospecificity of ketoreductase domains [1]. They may be used to reduce precursors of α-chloroalcohols, which are synthetic intermediates for various pharmaceutical compounds [2].

Biochem/physiol Actions

Ketoreductase, Product 77857, reduces 4-chloroacetoacetate with NADPH as a cosubstrate.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which reduces 1 μmol 4-chloroacetoacetate per minute at pH 6.0 and 25°C (cosubstrate NADPH).

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Automation Highlights from the Literature
Kerstin Thurow and Hilmar Weinmann
Journal of Laboratory Automation, 11, 1-6 (2006)
Roselyne Castonguay et al.
Journal of the American Chemical Society, 130(35), 11598-11599 (2008-08-13)
Tylactone synthase (TYLS) is a modular polyketide synthase that catalyzes the formation of tylactone (1), the parent aglycone precursor of the macrolide antibiotic tylosin. TYLS modules 1 and 2 are responsible for the generation of antidiketide and triketide intermediates, respectively
Guangming Xiong et al.
Chemico-biological interactions, 178(1-3), 215-220 (2008-11-13)
3Alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni is a key enzyme in the degradation of steroids in the environment. The encoding gene, hsdA, is expressed only at very low levels in the absence of steroids, but undergoes a several fold induction
Yongde Peng et al.
Zhonghua yi xue za zhi, 82(1), 31-34 (2002-04-16)
To investigate methodology of cloning full-length cDNA from tissues of normal adrenal gland and pheochromocytoma and predict their function. 104 samples from normal adrenal gland and 22 samples from pheochromocytoma were examined by expressed sequence tags (EST) sequencing, bioinformatics analysis
Jonathan A Doorn et al.
Biochemistry, 43(41), 13106-13114 (2004-10-13)
4-Oxonon-2-enal (4ONE) was demonstrated to be a product of lipid peroxidation, and previous studies found that it was highly reactive toward DNA and protein. The present study sought to determine whether carbonyl reductase (CR) catalyzes reduction of 4ONE, representing a

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