77398
(R)-Oxynitrilase, Linum usitatissimum, recombinant from Pichia pastoris
solution (clear), slightly yellow, ≥50 U/mL
Synonym(s):
(R)-Acetone cyanohydrin Lyase
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About This Item
MDL number:
UNSPSC Code:
12352204
recombinant
expressed in Pichia pastoris
form
solution (clear)
concentration
≥50 U/mL
color
slightly yellow
storage temp.
2-8°C
Biochem/physiol Actions
R-oxynitrilase catalyzes the enantioselective addition of HCN to aldehydes or ketones.
Components
contains ~20 mM sodium acetate, ~20 mM ZnCl2, ~1 M NaCl, pH ~5.7
Unit Definition
1 U corresponds to the amount of enzyme which cleaves 1 μmol acetone cyanhydrin to cyanide and acetone per minute at pH 4.2 and 30 °C
Other Notes
Investigation of the substrate range
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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J Albrecht et al.
Biotechnology and applied biochemistry, 17 ( Pt 2), 191-203 (1993-04-01)
The purification of (R)-oxynitrilase (EC 4.1.2.10) from Linum usitatissimum (flax) has been improved considerably. The enzyme is obtained from seedlings in 60% yield by fractional salt precipitation followed by ion-exchange and hydrophobic-interaction chromatography. Final gel-permeation chromatography yields a protein with
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