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Sigma-Aldrich

Protein Quantification Kit, wide range

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About This Item

UNSPSC Code:
12352200

usage

sufficient for 500 tests

shelf life

limited shelf life, expiry date on the label

shipped in

dry ice

storage temp.

2-8°C

General description

This kit is a complete, ready-to-use microtiter plate assay kit for the colorimetric detection of proteins. It is based on the reducing reaction of a tetrazolium salt under basic conditions. Tetrazolium salt is easily reduced with sugar residues of proteins and generates formazan dye. The maximum wavelength of the WST-8 formazan dye is 650 nm at pH 12.5.
The detection range of this protein quantification kit is from 50 μg/ml to 5000 μg/ml (incubation time 30 min).

Components

Contents: WST-8 Solution 10 ml, Buffer Solution 100 ml, BSA Standard Solution (10 mg/ml) 1.5 mL (500 tests)

Kit Components Only

Product No.
Description

  • WST-8 Solution 10 mL

  • Buffer Solution 100 mL

  • BSA Standard Solution (10 mg/ml) 1.5 mL

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Dario Lombardi et al.
Analytical and bioanalytical chemistry, 399(1), 347-352 (2010-11-05)
In this study, we give the proof of concept for a method to determine binding constants of compounds in solution. By implementing a technique based on magnetic beads with a microfluidic device for segmented flow generation, we demonstrate, for individual

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