75403

Crystallization Cryo Kit for Proteins

• Synonym(s): Protein Cryo Crystallography Kit
• UNSPSC Code: 12171500

Properties

• storage temp.: 2-8°C

Description

Application

The Crystallography Cryo Kit for Proteins provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.

Features and Benefits

• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, flow charts, and observation sheets
• Low incidence of undesirable solubility effects

Components

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.

Principle

Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization[1].

Kit Components Only

• Ammonium sulfate 0.17 M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% solution

Kit Components Also Available Separately

• 71591 Imidazole pH 6.5; 0.07 M - Sodium acetate 0.7 M - Glycerol 30% solution
• Sodium citrate tribasic pH 5.6; 0.07 M – Ammonium phosphate monobasic 0.7 M – Glycerol 30% (v/v) solution
• 72872 Magnesium chloride 0.18 M – HEPES-Na pH 7.5; 0.09M – 2-Propanol 27% (w/v) – Glycerol 10% solution
• 74613 Ammonium acetate 0.16M – TRIS hydrochloride pH 8.5; 0.08M – 2-Propanol 24% (v/v) – Glycerol 20% (v/v) solution
• Magnesium chloride 0.2M – HEPES Na pH 7.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution
• HEPES Na pH 7.5; 0.065M – Potassium sodium tartrate 0.52M – Glycerol 35% (v/v) solution
• Ammonium acetate 0.17M – Sodium acetate pH 4.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution
Magnesium acetate 0.2M – Sodium cacodylate pH 6.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution
Sodium citrate tribasic 0.2M – TRIS hydrochloride pH 8.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution
80074 Ammonium sulfate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution
Lithium sulfate 0.17 M – TRIS hydrochloride pH 8.5; 0.085M – Polyethylen glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution
Magnesium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% solution
80384 Sodium citrate tribasic 0.14M, HEPES Na pH 7.5; 0.07M, 2-Propanol 14% (v/v), Glycerol 30% solution
80387 Sodium acetate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution
Calcium chloride 0.19 M – HEPES-Na pH 7.5; 0.095M – Polyethylene glycol 400; 26.6% (w/v) – Glycerol 5% solution
HEPES-Na pH 7.5; 0.085M – Polyethylene glycol 400 1.7% (v/v) – Ammonium sulfate 1.7M – Glycerol 15% (v/v) solution
HEPES-Na pH 7.5; 0.075M – Sodium phosphate monobasic 0.6M – Potassium phosphate monobasic 0.6M – Glycerol 25% (v/v) solution
Lithium sulfate 0.4 M – Polyethylene glycol 8000 12% (w/v) – Glycerol 20% (v/v) solution
81892 Sodium formate 3.6 M – Glycerol 10% solution
Sodium acetate pH 4.6; 0.07M – 1.4 M Sodium formate – Glycerol 30% (v/v) solution
HEPES-Na pH 7.5; 0.085M – 2-Propanol 8.5% (v/v) – Polyethylene glycol 4000 17% (w/v) – Glycerol 15% (v/v) solution
83196 Sodium acetate pH 4.6; 0.08 M – Ammonium sulfate 1.6 M – Glycerol 20% (v/v) solution
Potassium phosphate monobasic 0.04M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% (v/v) solution
TRIS hydrochloride pH 8.5; 0.08 M – Ammonium phosphate monobasic 1.6 M – Glycerol 20% (v/v) solution
89252 Ammonium sulfate 1.5 M – Glycerol 25% solution
HEPES-Na pH 7.5; 0.09M – Sodium citrate tribasic 1.26M – Glycerol 10% solution
Polyethylene glycol 1500 24% (w/v) – Glycerol 20% (v/v) solution
91638 Magnesium formate 0.1M – Glycerol 50% (v/v) solution
Calcium chloride 0.02 M – Sodium acetate 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution
Sodium citrate tribasic 0.2M – HEPES-Na pH 7.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution
TRIS hydrochloride pH 8.5; 0.075 M – Ammonium sulfate 1.5 M – Glycerol 25% (v/v) solution
Ammonium phosphate monobasic 0.26 M – Glycerol 35% (v/v) solution
96864 Potassium sodium tartrate 0.26 M – Glycerol 35% (v/v) solution
Calcium chloride 0.14M – Sodium acetate pH 4.6; 0.07M – 2-Propanol 14% (v/v) – Glycerol 30% (v/v) solution
Sodium cacodylate pH 6.5; 0.07M – Sodium acetate 0.98M – Glycerol 30% (v/v) solution
Ammonium sulfate 0.16 M – Sodium acetate pH 4.6; 0.08M – Polyethylene glycol 4000 20% (w/v) – Glycerol 20% (v/v) solution
80084 HEPES Na pH 7.5; 0.075M – Lithium sulfate 1.125M – Glycerol 25% (v/v) solution

See All (37)

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H225,H315,H318,H336,H350,H360D,H411
• pcodes: P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids