The solubility of this product has not been determined. However, various sources state that this material is soluble in DMSO and only sparingly soluble in water. The exact concentrations are not reported.
73144
Resorufin
95%
Synonym(s):
7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
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20 MG
$152.00
About This Item
Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
Beilstein/REAXYS Number:
174850
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
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Quality Level
assay
≥95.0% (HPLC)
95%
form
solid
mp
>300 °C (lit.)
storage temp.
−20°C
SMILES string
O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
InChI
1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
InChI key
HSSLDCABUXLXKM-UHFFFAOYSA-N
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General description
Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.[1]
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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