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Sigma-Aldrich

LUCY® 506 solution

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About This Item

UNSPSC Code:
12171500

form

liquid

λmax

~495 nm in methanol

fluorescence

λex 504 nm; λem 515 nm in aqueous buffer pH 8.3 with SDS and BSA

storage temp.

2-8°C

Application

LUCY 506 is a fluorescent stain for protein electrophoresis, with high sensitivity and easy, fast and robust staining procedure for all kinds of SDS gels. Protein staining by LUCY 506 does not interfere with subsequent MALDI-MS.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

LUCY 506 is provided as a 5000× stock solution in DMF (5 mg/ml).

Analysis Note

Sensitivity of routine procedure is ca. 3 ng/band, with linear response between 3 and 1000 ng/band.

Legal Information

LUCY is a registered trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

136.4 °F

flash_point_c

58 °C

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Certificates of Analysis (COA)

Lot/Batch Number
1419642
1378193

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Questions

1–4 of 4 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. Can LUCY stains be used for quantification in solution. What is the linear range?

    1 answer

    1. LUCY 506 and LUCY 569 can be used to quantify proteins in solution.  They are applicable for 2 different protein concentrations (the linear range 0-50 ug/mL and 10-100 ug/mL).  Requirements are only buffer, SDS, and the LUCY dyes. Samples can be measured immediately after mixing.

      Helpful?

  3. Do the LUCY stains contain heavy metal?

    1 answer

    1. No, the LUCY stains do not contain a heavy metal.

      Helpful?

  4. What are the detection limits for LUCY stains?

    1 answer

    1. LUCY 506 shows highest sensitivity, with a detection limit of about 3 ng per band. The standard procedure is a post-electrophoretic stain with omission of the fixation step.

      Helpful?

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