Journal of biotechnology, 16(3-4), 233-243 (1990-11-01)
Large-scale purification of a Dictyostelium discoideum cell surface glycoprotein, which is anchored in the membrane via a glycosylphosphatidylinositol (GPI) moiety, is described. The purification protocol involved four steps: separation of crude cell membranes by low-speed centrifugation, delipidization of these membranes
Use of n-Octyl-b-D-thioglucopyranoside in the isolation of a bacterial membrane protein: an improved method for immunological applications.
Peula-Garcia, J.M., et al.
Journal of Surface Science and Technology, 22(3-4), 141-157 (2006)
Protein expression and purification, 65(2), 204-213 (2009-03-20)
Structure determination of G protein-coupled receptors is still in its infancy and many factors affect whether crystals are obtained and whether the diffraction is of sufficient quality for structure determination. We recently solved the structure of a thermostabilised turkey beta
Mouse liver microsomes treated with octylthioglucoside were examined for iron-stimulated ATPase activity. The activity was about 6 mumol Pi/mg protein/hr under optimal conditions [300 mM KC1, 3 mM MgSO4, 10 mM glutathione(GSH), 100 microM FeCl3, 3 mM ATP and 50
Antimicrobial agents and chemotherapy, 56(4), 1735-1743 (2012-02-01)
The antigen 85 (Ag85) protein family, consisting of Ag85A, -B, and -C, is vital for Mycobacterium tuberculosis due to its role in cell envelope biogenesis. The mycoloyl transferase activity of these proteins generates trehalose dimycolate (TDM), an envelope lipid essential
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