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biological source
human
reprogramming method
retrovirus
description
age (N/A)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
frontotemporal dementia
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Depositor
Sigma-Aldrich
Sigma-Aldrich
Cell Line Description
Derivation
Primary cell type: -
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3 Yes
TRA 1-60 Yes
POU5F1 (OCT-4) Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Karyotyping method: G-Banding
Genetic Modification
Disease/phenotype related modifications
Disease: Frontotemporal dementia
Type of modification: Isogenic
Chromosome location: 17q21.31
Target locus modification: Mutated
Primary cell type: -
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3 Yes
TRA 1-60 Yes
POU5F1 (OCT-4) Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Karyotyping method: G-Banding
Genetic Modification
Disease/phenotype related modifications
Disease: Frontotemporal dementia
Type of modification: Isogenic
Chromosome location: 17q21.31
Target locus modification: Mutated
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Legal Information
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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