66540367

BIONi010-C-5

Human iPS Cell Line

• UNSPSC Code: 41106509

Properties

• biological source: human
• reprogramming method: episomal
• description: age (N/A)
• manufacturer/tradename: EBiSC^™
• gender: male
• growth mode: adherent (pluripotent)
• technique(s): cell culture | stem cell: suitable
• relevant disease(s): Alzheimer′s disease
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Bioneer

Cell Line Description

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NEUROD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
FOXA2 Yes
GATA6 Yes
GSC Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
DCN Yes
Vimentin Yes
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: 46 XY
Image / Result

STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Isogenic
Description: Bioneer to update this information when characterization has been completed. CD33 gene mutated; modified sequence CCTGCTGTGGGCAGgtgagtggctcgggggagcttgaccagaggttgatcttctct

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: Essential E8^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 18%
Temperature: 37°C

Legal Information

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable