66540266

BIONi015-A

Human iPS Cell Line

• Synonym(s): EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
• UNSPSC Code: 41106509

Properties

• biological source: human adipose-derived MSC
• reprogramming method: episomal
• description: age (20-24)
• manufacturer/tradename: EBiSC^™
• gender: male
• growth mode: adherent (pluripotent)
• technique(s): cell culture | stem cell: suitable
• relevant disease(s): none
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Bioneer

Cell Line Description

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TDG1 (+)SSEA-1(-)SSEA-4(+)TRA 1-81(+)SOX2 (+)DMNT3B(+GDF3(+)GABRB3(+)NANOG(+)POU5F1 (OCT-4) (+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: Essential E8^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 5%
Temperature: 37°C

Legal Information

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable