66540177

UNEWi022-A

Human iPS Cell Line

• Synonym(s): EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
• UNSPSC Code: 41106509

Properties

• biological source: human dermis (fibroblast)
• reprogramming method: Sendai virus
• description: age (85-89)
• manufacturer/tradename: EBiSC^™
• gender: male
• growth mode: adherent (pluripotent)
• technique(s): cell culture | stem cell: suitable
• relevant disease(s): age-related macular degeneration
• shipped in: dry ice
• Storage temp.: −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
University of Newcastle

Cell Line Description

Derivation
Primary Cell Type: Fibroblast of dermis
Developmental Stage: Adult
Passage Number: P4
Reprogramming Method: Sendai Virus (KLF4, MYC, POU5F1, SOX2)
Other: From the same donor as UNEWi022-B, UNEWi022-C

Characterization
Marker Expression: NANOG(+), TRA160(+), SSEA4(+), SSEA1(-)
Differentiation Potential: Ectoderm (TUBB3+), Endoderm (AFP+), Mesoderm (ACTA2+)

Microbiology / Virus Screening
HIV1: Negative
HIV2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Microbial Growth: None Detected
Viability: Viable Post-Cryopreservation

Genotyping
Karyotype: P14, Chromosome 12 gain by molecular karyotype SNP array
Genotype: 16 allele STR profile available upon request

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: mTeSR^®
Passage method: EDTA
Matrix: Matrigel^®/Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 20%
Temperature: 37°C

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable