66540136
WTSIi066-A
Human iPS Cell Line
Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
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About This Item
UNSPSC Code:
41106509
biological source
human dermis (fibroblast)
Quality Level
reprogramming method
Sendai virus
description
age (N/A)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
none
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Depositor
Wellcome Trust Sanger Institute
Wellcome Trust Sanger Institute
Cell Line Description
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi066-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG (+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 20.188
Novelty score: 1.416
Microarray data: https://www.ebi.ac.uk/ega/datasets/EGAD00010000775
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi066-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG (+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 20.188
Novelty score: 1.416
Microarray data: https://www.ebi.ac.uk/ega/datasets/EGAD00010000775
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine
Medium: -
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°C
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°C
Legal Information
EBiSC is a trademark of Fraunhofer-Gesellschaft
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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