66540122
WTSIi047-B
Human iPS Cell Line
Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
About This Item
biological source
human dermis (fibroblast)
Quality Level
reprogramming method
Sendai virus
description
age (55-59)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
none
shipped in
dry ice
storage temp.
−196°C
General description
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Wellcome Trust Sanger Institute
Cell Line Description
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi047-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 18.05
Novelty score: 0.917
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266840
Linkage
Subculture Routine
Passage method: Enzyme-free cell dissociation / EDTA
CO2 concentration: 5%
Culture medium: TeSR® E8®
Legal Information
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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