Regarding the extreme thickness of the tissue, the staining intensity may be influenced by the amount of nuclear matter present. The Nuclear Fast Red solution, also known as Kernechtrott solution, requires careful preparation and staining steps. Incomplete staining may result from inadequate boiling of the solution or excessive dilution, affecting the concentration of nuclear fast red and aluminum sulfate. It is important to follow the standard procedures for preparing and using the Nuclear Fast Red solution to achieve the intended staining.
60700
Nuclear Fast Red
suitable for microscopy
Synonym(s):
4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
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$161.00
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$456.00
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5 G
$161.00
25 G
$456.00
About This Item
Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
Molecular Weight:
357.27
Colour Index Number:
60760
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
form
powder
Quality Level
technique(s)
microbe id | staining: suitable
solubility
methanol: water (1:1): 0.01 g/10 mL, red
εmax
≥275 at 550-560 nm in DMSO
≥300 at 516-526 nm in DMSO
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI
1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
InChI key
IFSXZLJQEKGQAF-UHFFFAOYSA-M
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General description
Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.
Application
Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:
- staining sperm and spermatids in semen
- identifying areas for PCR analysis in paraffin-embedded tissues
- nuclear counterstaining following immunostaining and silver staining
Used as a biological stain
Biochem/physiol Actions
Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.[1]
Principle
Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain® can also be used fornuclear staining with alcian blue.
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Nuclear Fast Red solution has been staining the entire tissue, not just the nucleus. How to troubleshoot this issue effectively?
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