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60142

Sigma-Aldrich

Potassium chloride solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

KCl, potassium chloride

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About This Item

Linear Formula:
KCl
CAS Number:
7447-40-7
Molecular Weight:
74.55
Beilstein/REAXYS Number:
1711999
MDL number:
MFCD00011360
UNSPSC Code:
12352302
PubChem Substance ID:
57651726
NACRES:
NA.26

grade

for molecular biology

product line

BioUltra

concentration

~1 M in H2O

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

density

1.04 g/mL at 20 °C

anion traces

sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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General description

Potassium is an intracellular cation with just 2% of total body K+ inhabiting the extracellular fluid. The distribution of K+ between the intracellular and extracellular space is analyzed by the action of several transport pathways like Na+-K+-ATPase, the Na+-K+-2Cl-cotransporter NKCC1, the four KCCs, and a plethora of K+ channels. Also, skeletal muscle comprises almost 75% of total body potassium and elicits considerable influence on extracellular K+. Potassium chloride is consumed orally to prevent or correct potassium depletion caused by diuretic use or other conditions. It is used as a food supplement and salt substitute.

Application

Potassium chloride solution has been used as a component of:
  • multiple annealing and looping based amplification cycles (MALBAC) lysis buffer to digest proteins for single-cell multiomics sequencing
  • single-cell tagged reverse transcription (STRT) buffer, and STRT-RT buffer for single-cell RNA-sequencing
  • phosphate buffer saline (PBS) to wash transfected cells

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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