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54295

Sigma-Aldrich

Hydroxyethyl-cellulose dyed with ostazin brilliant red H-3B

10% dye basis

Synonym(s):

OBR-Hydroxyethyl-cellulose, Ostazin brilliant red H-3B hydroxyethyl-cellulose

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

form

fibers

concentration

10% (dye)

Other Notes

Soluble chromogenic substrate for the assay of endo-1,4-(beta)-glucanases[1]; Substrate used for the detection of endo-1,4-(beta)-glucanases in gels[2]; Substrate used in agar nutrient medium for the detection of microbial producers of endo-1,4-(beta)-glucanase[3]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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P Biely et al.
Analytical biochemistry, 144(1), 147-151 (1985-01-01)
A simple, highly sensitive zymogram technique for detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in polyacrylamide gels after electrophoresis or isoelectric focusing was developed. The detection employs transparent agar replicas containing soluble covalently dyed polysaccharides, hydroxyethylcellulose dyed with Ostazin brilliant red H-3B
V. Farkas et al.
FEMS Microbiology Letters, 28, 137-137 (1985)
P Biely et al.
Analytical biochemistry, 144(1), 142-146 (1985-01-01)
New soluble chromogenic substrates were prepared for specific and rapid assays of endo-1,4-beta-xylanases and endo-1,4-beta-glucanases. A soluble beechwood 4-O-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulose was coupled to Ostazin brilliant red H-3B. The assays are based on

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