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53403

Sigma-Aldrich

Atto 532 alkyne

BioReagent, suitable for fluorescence

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

product line

BioReagent

form

solid

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 532 nm; λem 553 nm±10 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus Atto 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm.
A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.
The alkyne modification is used in the Huisgen reaction (“Click Chemistry“).

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

pictograms

Exclamation mark

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Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B.; et al
Optical Nanoscopy, 1(1), 3-3 (2012)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)

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