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53113

Sigma-Aldrich

Purine nucleoside phosphorylase

recombinant, expressed in E. coli

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About This Item

CAS Number:
9030-21-1
Enzyme Commission number:
2.4.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00131739
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

liquid

specific activity

≥9 U/mL

storage temp.

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

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Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism[1]. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations. Product 53113 is recombinant and expressed in E. coli.

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine[1]. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency[2][3].

Unit Definition

1 U will cause the phosphorolysis of 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBP0596V
BCBD6983V
BCBD6983
BCBD5814
BCBC3774

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Purine Nucleoside Phosphorylase Deficiency in a Patient With Spastic Paraplegia and Recurrent Infections
Ferda Ozkinay, Sacide Pehlivan, et al.
BMJ Case Reports, 22, 741-743 (2007)
Purine nucleoside phosphorylase deficiency with a novel PNP gene mutation: a first case report from India
Manisha Rajan Madkaikar, et al.
BMJ Case Reports, DOI:10-DOI:10 (2011)
Fernanda Canduri et al.
Biochemical and biophysical research communications, 313(4), 907-914 (2004-01-07)
Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP
Joseph T P Yeeles et al.
Nucleic acids research, 39(6), 2271-2285 (2010-11-13)
The oligomeric state of Superfamily I DNA helicases is the subject of considerable and ongoing debate. While models based on crystal structures imply that a single helicase core domain is sufficient for DNA unwinding activity, biochemical data from several related
Shanta Bantia et al.
Current opinion in drug discovery & development, 7(2), 243-247 (2004-12-18)
Purine nucleoside phosphorylase (PNP)-deficient children exhibit profound impairment in the T-cell component of their immune systems, but have normal B-cell function. This rare condition provides a model for the development of specific inhibitors of PNP, which should enable selective suppression
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