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51153

Sigma-Aldrich

LUCY® Starter Kit

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About This Item

UNSPSC Code:
12352200

storage temp.

2-8°C

Application

The LUCY Starter Kit contains three fluorescent protein stains for SDS-PAGE gels.
  • LUCY 506 for high sensitivity staining with a detection limit of 3 ng protein per band. Staining is complete after 60 minutes and detection is possible immediately thereafter.
  • LUCY 565 is recommended for staining SDS-PAGE gels prior to Western blot transfer. With traditional staining, 2 separate gels are required and therefore, double the amount of sample is necessary.
  • LUCY 569 is particularly well suited for protein quantitation, displaying a broad, linear, dynamic range between 10–6,000 ng protein per band.

Legal Information

LUCY is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • LUCY® 565 solution 50 μL

Kit Components Also Available Separately

Product No.
Description
SDS
  • LUCY® 506 solution 50 μL
  • LUCY® 569 solution 50 μL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCBP1551V
BCBN5083V
BCBH7340V
BCBB1545
1436044

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Biji T Kurien et al.
Methods in molecular biology (Clifton, N.J.), 869, 403-405 (2012-05-16)
Gel electrophoresis is an important methodology employed for protein analysis. It is often necessary to elute and recover proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The procedure involves localizing the protein of interest on the gel following
Yi Hu et al.
Molecular biotechnology, 28(1), 63-76 (2004-10-01)
This review focuses on recent developments in gel-based proteomics techniques. By combining traditional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoretic techniques with recent advances in protein labeling using different classes of molecules (i.e., fluorescent dyes, chemical probes

Questions

1–4 of 4 Questions  

  1. Do the LUCY stains contain heavy metal?

    1 answer

    1. No, the LUCY stains do not contain a heavy metal.

      Helpful?

  2. Can LUCY stains be used for quantification in solution. What is the linear range?

    1 answer

    1. LUCY 506 and LUCY 569 can be used to quantify proteins in solution.  They are applicable for 2 different protein concentrations (the linear range 0-50 ug/mL and 10-100 ug/mL).  Requirements are only buffer, SDS, and the LUCY dyes. Samples can be measured immediately after mixing.

      Helpful?

  3. What are the detection limits for LUCY stains?

    1 answer

    1. LUCY 506 shows highest sensitivity, with a detection limit of about 3 ng per band. The standard procedure is a post-electrophoretic stain with omission of the fixation step.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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