42605
FragIT™ MidiSpin Kit
for cleaving and purifying up to 10mg IgG
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About This Item
UNSPSC Code:
12352204
storage temp.
2-8°C
General description
1 ml settled resin of FabRICATOR (IdeS) immobilized on highly cross-linked agarose and 1 ml CapureSelect IgG-Fc affinity ligand immobilized on agarose. Agarose resins are suspended in 20% ethanol/water (V/V)
Application
FragIT™ MidiSpin Kit (cleaving and purifying up to 10mg IgG) can be used for antibody characterization.
Biochem/physiol Actions
IgG fragmentation is accompanying with adverse consequences like low yield, time consuming optimization and operation at low pH with loss of immunoreactivity. These consequences can be overcome with FragIT™ MidiSpin Kit, which comprises of pre-filled Fabricator enzyme covalently coupled to agarose beads that facilitates the cleavage of IgG (cleaving and purifying up to 10mg IgG) to generate pure F(ab′)2 and Fc fragments. FabRICATOR is a digestive enzyme that digests IgG specifically below the hinge region so there is no risk of over digestion if incubation time exceeds.
Unit Definition
1 ml of settled resin fragments ≥ 95% of 5 mg of human IgG in 15 minutes at 25°C, pH 6.6 as monitored by SDS-PAGE.
Analysis Note
Enzymatic activity: corresponds to requirements
Legal Information
FragIT is a trademark of Genovis AB
signalword
Danger
hcodes
Hazard Classifications
Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk_germany
WGK 3
flash_point_f
57.2 °F - closed cup
flash_point_c
14.0 °C - closed cup
Certificates of Analysis (COA)
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Lukmanee Tradtrantip et al.
Molecular pharmacology, 83(6), 1268-1275 (2013-04-11)
Neuromyelitis optica (NMO) is an inflammatory demyelinating disease of the central nervous system caused by binding of pathogenic IgG autoantibodies (NMO-IgG) to astrocyte water channel aquaporin-4 (AQP4). Astrocyte damage and downstream inflammation require NMO-IgG effector function to initiate complement-dependent cytotoxicity
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