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42395

Sigma-Aldrich

Tannase from Aspergillus ficuum

powder, white, ≥150 U/g

Synonym(s):

Tannin acyl Hydrolase

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250 MG
$262.00
1 G
$862.00

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Available to ship onMay 01, 2025Details



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250 MG
$262.00
1 G
$862.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

$262.00


Available to ship onMay 01, 2025Details


biological source

Aspergillus sp. (A. ficuum)

Quality Level

form

powder

specific activity

≥150 U/g

impurities

25 mM potassium phosphate
250 mM NaCl
50% glycerol

color

white

storage temp.

2-8°C

General description

Tannase is an enzyme that is produced by several organisms such as plants, bacteria, fungi, and yeast. This enzyme is also found in plant sources such as tannin-rich vegetables, especially in the leaves, fruits, branches, and bark.

Application

Tannase from Aspergillus ficuum has been used:
  • as a standard to determine the tannase activity of bacterial isolates
  • to study its effects on the inhibitory activity of tannic acid on biofilm formation
  • to obtain Proanthocyanidins (PA) by enzymatic hydrolysis of grape skin and seeds

Biochem/physiol Actions

Tannase catalyzes the ester bonds located in complex tannins, gallo-tannins, and gallic acid esters, which results in the release of gallic acid. This enzyme can be used as a clarifying agent in the manufacture of beer, tea, wine, and juices and to treat tannin-polluting agricultural waste and industrial effluents.

Unit Definition

1 U corresponds to the amount of enzyme which changes the absorbance at 310 nm by 1.0 per minute at pH 4.7 and 30°C (tannic acid as substrate, final volume 3 ml)

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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I Darah et al.
Applied biochemistry and biotechnology, 165(7-8), 1682-1690 (2011-09-29)
Agitation speed was found to influence the tannase production and fungal growth of Aspergillus niger FETL FT3. The optimal agitation speed was at 200 rpm which produced 1.41 U/ml tannase and 3.75 g/l of fungal growth. Lower or higher agitation
Amrita Banerjee et al.
The protein journal, 31(4), 306-327 (2012-03-31)
The tannase protein sequences of 149 bacteria and 36 fungi were retrieved from NCBI database. Among them only 77 bacterial and 31 fungal tannase sequences were taken which have different amino acid compositions. These sequences were analysed for different physical
Erik Böer et al.
Applied microbiology and biotechnology, 92(1), 105-114 (2011-05-12)
Tannase (tannin acyl hydrolase, EC 3.1.1.20) hydrolyses the ester and depside bonds of gallotannins and gallic acid esters and is an important industrial enzyme. In the present study, transgenic Arxula adeninivorans strains were optimised for tannase production. Various plasmids carrying
Kevin D Kohl et al.
Environmental microbiology, 18(6), 1720-1729 (2015-03-11)
It has been hypothesized that herbivores host tannin-degrading bacteria (TDB) to overcome the toxic challenges posed by plant tannins. While TDB have been isolated from the guts of numerous mammals, their functional significance to their hosts has never been explicitly
S P Beena et al.
Natural product communications, 6(12), 1933-1938 (2012-02-09)
Garcinia gummi-gutta (syn. G. cambogia, G. quaesita), known to have medicinal properties, was evaluated as a substrate and inducer for tannase production by a marine Aspergillus awamori BTMFW032, under slurry state fermentation using Czapekdox-minimal medium and sea water as the

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