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41493

Sigma-Aldrich

Savinase® CLEA

≥8 U/g

Synonym(s):

Protease from Bacillus species, cross-linked enzyme aggregate

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About This Item

UNSPSC Code:
12352200
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Quality Level

100

specific activity

≥8 U/g

General description

Savinase® a globular protein, is a serine protease that contains hydrophobic residues[1] and metal-binding sites for Na+ and Ca+.[2] 

Application

Savinase® CLEA may be used as a serine protease for the proteolytic lysis of P. aeruginosa and S. aureus biofilms.[3] It has also been used to treat S. cerevisiae cell wall samples for extraction of glycans.[4]

Biochem/physiol Actions

Savinase® is a serine endopeptodase, which is responsible for stereoselective hydrolysis of amino esters and selective esters. It is mainly required for the hydrolysis of proteins and is used in transesterification and transpeptidation.
Savinase® stereoselective hydrolysis of selective as well as amino esters.[5] It is bactericidal towards S. aureus strains.[6] Savinase is stable in the pH range 7-10 and has potential industrial application for use in detergents.[1]

Unit Definition

1 U corresponds to the amount of enzyme which sets free 1 μmol folin-positive amino acids and peptides (as tyrosine) per minute at pH 8.0 and 37°C (casein as substrate)

Legal Information

Savinase is a registered trademark of Novozymes Corp.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCBR7320V
BCBP3481V
BCBK3019V
BCBG3595V
BCBC2266V

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Protease from Bacillus sp. liquid, ≥8 U/g

Sigma-Aldrich

P5860

Protease from Bacillus sp.

Adak Nasiripourdori et al.
International journal of biological macromolecules, 44(4), 311-315 (2008-10-29)
The industrial utilization of savinase is mainly constrained by its stability limitations. In the present study, the irreversible thermoinactivation of savinase has been evaluated at 70 degrees C, and various possible mechanisms for irreversible thermoinactivation of savinase were examined. The
Abdul Razzaq et al.
Frontiers in bioengineering and biotechnology, 7, 110-110 (2019-07-03)
The use of chemicals around the globe in different industries has increased tremendously, affecting the health of people. The modern world intends to replace these noxious chemicals with environmental friendly products for the betterment of life on the planet. Establishing
Savinase is a bactericidal enzyme.
Edward M Smith et al.
Applied and environmental microbiology, 69(1), 719-720 (2003-01-07)
Elodie Lefebvre et al.
International journal of antimicrobial agents, 48(2), 181-188 (2016-07-19)
A multistep strategy was used to generate a combined antibiofilm treatment that could efficiently decrease the biomass of dense biofilms (≥6 × 10(7) CFU/cm(2)). Several compounds that exhibited activity against various targets were tested individually and in combination to search for possible synergistic
Shanshan Wu et al.
PeerJ, 8, e9408-e9408 (2020-07-04)
Several examples have emerged of enzymes where slow conformational changes are of key importance for function and where low populated conformations in the resting enzyme resemble the conformations of intermediate states in the catalytic process. Previous work on the subtilisin
View All Related Papers

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