17490

Bromocresol Purple

suitable for microscopy

• Synonym(s): 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Empirical Formula (Hill Notation): C₂₁H₁₆Br₂O₅S
• CAS Number: 115-40-2
• Molecular Weight: 540.22
• Beilstein/REAXYS Number: 359618
• EC Number: 204-087-8
• MDL number: MFCD00011681
• UNSPSC Code: 12171500
• PubChem Substance ID: 57647609
• NACRES: NA.47

Properties

• form: powder
• technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• mp: 240 °C (dec.) (lit.)
• solubility: hot ethanol: 0.1 g/10 mL
• εmax: ≥400 at 414-424 nm in methanol
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• InChI key: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8).[1] It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.[2]

Application

Spectrophotometric determination of serum-albumin

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves