In order to retain the maximum potency of EGF it is best to store the reconstituted growth factor at 1mg/mL or with a carrier such as BSA at 0.1%, for lower concentrations. Solutions are stable up to 1 week when stored refrigerated. One time use aliquotes may also be stored frozen at -20 deg. C. Avoid repeated freeze/thaw cycles. For an example of product stability for EGF see the link below to an EGF product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/119/512/e5160dat.pdf
131-500A
Keratinocyte Serum-Free Growth Medium for adult cells (500 ml)
Synonym(s):
Primary Cell Culture Media
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Recommended Products
form
liquid
shelf life
6 mo.
manufacturer/tradename
Cell Applications, Inc
shipped in
wet ice
storage temp.
2-8°C
General description
All-in-one ready-to-use media
application
Keratinocyte Serum-Free Growth Medium for adult cells (500 ml) has been used in the culturing of human bronchial epithelial cells.[1]
Quality
Each lot was tested for the ability to support plating, spreading and proliferation
Preparation Note
contained in protocol
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
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Protocol said that we have to add EGF just before using it for cell culture? Every time we have to add EGF before transferring the medium to keratinocytes. Can't we add it to a 50 ml aliquot and store it at 4 degrees for its use within a week or 10 d?
1 answer-
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Has this product already been supplemented with recombinant human epidermal growth factor (rhEGF) and bovine pituitary extract (PE)? Or 133-GSA needs be supplemented?
1 answer-
Keratinocyte Serum-Free Growth Medium for adult cells (500 ml) is a all-in-one ready-to-use media. Please see the product protocol where no supplementation is recommended: https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/primary-cell-culture/human-epidermal-keratinocytes.
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