Please consider using DMEM from cat#D6546, recommended by the European Collection of Authenticated Cell Cultures (ECACC). This product has a different formulation from D6429 as it contains L-Glutamine in addition to the 2mM Glutamine + 10% FBS recommended to add to DMEM. For more information on the composition of D6546 and D6429, please refer to the following web page: Composition of D6546 and D6429. If using D6429 instead of D6546, it is advisable to consider adding more L-Glutamine as recommended by ECACC. For a list of product numbers, please consult page 66 of 67 in the ECACC lab handbook, available at the following link: ECACC Lab Handbook.
08062601
F11 cell line from rat
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym(s):
F-11 Cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Pricing and availability is not currently available.
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General description
For complete product information, please see ECACC
Cell Line Origin
A somatic cell hybrid of a rat embryonic dorsal root ganglion (DRG) and mouse neuroblastoma cell line N18TG2. The line retains both rat and mouse chromosomes and synthesises both rat and mouse isoenzymes. Manifests a unique combination of action potential properties and cell surface markers.
Application
Recommended culture medium: DMEM + 2mM Glutamine + 10% FBS
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Are there specific requirements for the DMEM variation needed for culturing cells if the cells do not appear healthy after thawing and plating for the first time? I purchased D6429 (Sigma) for this purpose, but the next day, most cells were floating and the ones that were attached did not look 100% healthy. I'm uncertain if it's due to this batch of cells or if the media is off.
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