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02816

Sigma-Aldrich

Europium-tetracycline complex reagent

Synonym(s):

Eu3Tc, EuTc

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About This Item

UNSPSC Code:
12352200

form

powder

fluorescence

λex 386 nm; λem 617 nm±5 nm (H2O2-bound)

storage temp.

−20°C

Application

The dye europium-tetracycline (EuTc) is ideal for use in assays for H2O2 and peroxide-related enzymes. EuTc alone is weakly fluorescent but combines rapidly with free H2O2 to form a EuTc-H2O2 complex that is 15-fold brighter than EuTc alone. Measuring fluorescent intensity makes it possible to quantify the amount of H2O2 present in a sample.

Principle

The basic principle of the assay is based upon the following equilibrium:
EuTc (weakly fluorescent) + H2O2 ↔ EuTc-H2O2 (strongly fluorescent)

pictograms

Exclamation mark

signalword

Warning

pcodes

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Skin Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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