MIC027

Pseudomonas Aeruginosa - Presence/Absence 10 Sample Set

proficiency testing material

• UNSPSC Code: 12352200

Properties

• packaging: set of 10 ea
• storage temp.: −20°C

Description

General description

This proficiency testing sample is produced in accordance with ISO/IEC 17043:2010. All information regarding the use of this material can be found in the reporting packet supplied for each sample.

Preparation Note

1) This sample set contains 10 vials each containing a dehydrated tablets containing possible organisms. Ten sterile 100 mL phosphate buffered hydrolyzing solutions are also provided. Each sample vial is uniquely marked with a sample number and color coded cap (Samples 1, 6 - Clear, Sample 2, 7 - Red, Sample 3, 8 - Blue, Sample 4, 9 - Green, Samples 5, 10 - Yellow.) The buffer bottles are NOT labeled. Remember to label the buffer bottles prior to mixing. The vials containing the dehydrated sample must be stored at Store at 4°C. The sample is the colored disk. If the disk is lodged in the cap, drop entire cap into the hydration fluid. Ensure the disk has dissolved completely (no color remains) and aseptically remove the cap prior to analysis. The hydrolyzing fluid may be stored at room ! temperatures. The bacteria are provided in dehydrated form for enhanced stability. They must be re-hydrated prior to performing the tests. The vials holding the bacteria are sterile in the sealed box. Once the box is opened it is recommended hydration and analysis is started immediately. Each positive disk contains a concentration of bacteria between 20 - 1000 CFUs. Before you begin, make certain that appropriate methods and media are being used. Label each dilution bottle with the sample number, the study number prior to hydrolyzing the samples. All of these numbers can be found on the vial labels. Make sure to use a permanent marker that is water insoluble. Do not transfer any disks into hydrolyzing liquid prior to warming it. If you are using an enzyme substrate test follow manufacturer¢s instructions. If the result is questionable at the end of the incubation period, you may incubate for an additional 4 hours or as provided in the method. It is important that you repor! t values for all 10 samples. You must report all samples to receive an evaluation. We are not allowed to make any assumptions so please fill out your forms carefully.

Preparation Instructions
1) Remove the sample vials and dilution bottles from refrigeration and allow them to equilibrate to room temperature. Open the bottle and aseptically transfer a dehydrated tablet to the bottle. Label the bottle, cap and shake gently. The tablet will dissolve completely in about 15 minutes. Repeat the above procedure with each sample. Due to the sensitivity of the organisms, analysis should be completed with in 1 hour of hydration. Each sample may contain a concentration of bacteria from 20 to 1000 CFU/100mL. You may dilute the sample with phosphate buffer to meet your method needs.

Other Notes

Proficiency Testing Schedule (2016-2017)

Drinking Water
WS16-1 January 27 - March 11, 2016
WS16-2 April 6 - May 20, 2016
WS16-3 July 13 - August 26, 2016
WS16-4 September 28 - November 11, 2016

WS17-1 January 18 - March 3, 2017
WS17-2 March 29 - May 12, 2017
WS17-3 July 12 - August 25, 2017
WS17-4 September 27 - November 10, 2017

Quick-Turn Study - (non-scheduled proficiency test that is available at any time)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable