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G6639

Sigma-Aldrich

Guanidine thiocyanate

≥97% (titration)

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
Beilstein/REAXYS Number:
3563461
EC Number:
209-812-1
MDL number:
MFCD00013027
UNSPSC Code:
12352209
PubChem Substance ID:
24895264
NACRES:
NA.55

biological source

synthetic

Quality Level

200

assay

≥97% (titration)

form

powder or crystals

technique(s)

RNA extraction: suitable

color

white to off-white

mp

120-122 °C (lit.)

solubility

water: 4 M, clear, colorless

anion traces

chloride (Cl-): ≤2.5%

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Application

Guanidine thiocyanate has been used:
  • as a denaturing agent to study its effects on surface activity of globular protein
  • for the extraction of total RNA from primary hepatocytes
  • as a component of the incubating buffer for extraction of DNA from human bone
  • as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
  • as a component of lysis solution for extraction of RNA from rat pancreatic tissues
Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF2667V
WXBF2503V
WXBF3958V
WXBF1033V
WXBF2154V

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