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C0537

Sigma-Aldrich

5-Carboxyfluorescein

≥99% purity (HPLC), powder

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
Beilstein/REAXYS Number:
57037
MDL number:
MFCD00036874
UNSPSC Code:
12171500
PubChem Substance ID:
24892258
NACRES:
NA.47

Product Name

5-Carboxyfluorescein, ≥99% (HPLC)

Quality Level

200

assay

≥99% (HPLC)

form

powder

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.[1]

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH.[2] 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.[3]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000082408
0000156737
0000082408
0000133493

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Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis.
Zhang B, et al.
Journal of Lipid Research, 46, 2265-2265 (2005)
Christian Dittrich et al.
Macromolecular bioscience, 10(12), 1406-1415 (2010-12-18)
CD3ac, an uncharged and strongly hydrophobic 10 amino acid peptide (Ac-LK(Ac)-LK(Ac)-LK(Ac)-LW-DL-LW-DL-LW-DL-LW-NH2) was synthesized and purified. The peptide readily dissolves in ethanol and--upon solvent exchange to water--assembles into solid spherical particles with diameters of around 500 nm and low size-polydispersity. CD3ac
Peilin Yang et al.
Analytical chemistry, 77(8), 2482-2489 (2005-04-15)
Fluorescence anisotropy capillary electrophoresis (FACE) and affinity probe capillary electrophoresis (APCE) with laser-induced fluorescence detection were evaluated for analysis of peptide-protein interactions with rapid binding kinetics. The Src homology 2 domain of protein SH2-Bbeta (SH2-Bbeta (525-670)) and a tyrosine-phosphorylated peptide
M Goetz et al.
Endoscopy, 39(4), 350-356 (2007-04-12)
Although various improvements in tissue imaging modalities have recently been achieved, in-vivo molecular and subsurface imaging in the field of gastroenterology remains a technical challenge. In this study we evaluated a newly developed, handheld, miniaturized confocal laser microscopy probe for
Reeta Kangas et al.
Mechanisms of ageing and development, 143-144, 1-8 (2014-12-03)
Biological aging is associated with physiological deteriorations, which are partly due to changes in the hormonal profile. MicroRNAs regulate various processes associated with cell senescence; differentiation, replication and apoptosis. Serum microRNAs have potential to serve as noninvasive markers for diagnostics/prognostics
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