Item B3883 can be stored at room temperature.
B3883
Bile bovine
dried, unfractionated
Synonym(s):
Ox gall powder
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25 G
$68.80
100 G
$216.00
500 G
$502.00
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25 G
$68.80
100 G
$216.00
500 G
$502.00
About This Item
Recommended Products
sterility
non-sterile
Quality Level
form
dried, unfractionated
color
yellow-brown
solubility
water: soluble 50 mg/mL
application(s)
microbiology
General description
Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.
Application
Bile bovine has been used:
- in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition[1]
- as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology[2]
- as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583[3]
Biochem/physiol Actions
Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.[4]
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics
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PLoS ONE, 13(3), e0192964-e0192964 (2018)
Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties
Guo XH, et al.
Anaerobe, 16(4), 321- 326 (2010)
Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate
Solheim M, et al.
Applied and Environmental Microbiology, 73(18), 5767- 5774 (2007)
Helena Šeme et al.
Food research international (Ottawa, Ont.), 94, 45-53 (2017-03-16)
Malabsorption of dietary sugars is a common cause of gastrointestinal discomfort, affecting up to one in three people with debilitating symptoms, such as abdominal pain, osmotic diarrhoea, bloating and flatulence. Besides dietary interventions, it has been suggested that ingestion of
Impact of bile salt adaptation of Lactobacillus delbrueckii subsp. lactis 200 on its interaction capacity with the gut
Burns P, et al.
Research in Microbiology, 162(8), 782- 790 (2011)
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WHAT IS THE PROPER TEMPERATURE FOR STORAGE?
1 answer-
Helpful?
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Can you tell us the exact bile salt concentration?
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.
Helpful?
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I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.
Helpful?
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