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89554

Sigma-Aldrich

TNT buffer solution

BioUltra, Molecular Biology, pH 8.0

Synonym(s):

TRIS NaCl Tween® 20 buffer solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

Molecular Biology

Quality Level

100

product line

BioUltra

form

liquid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.03

Application

TRIS NaCl Tween 20 (TNT) buffer solution, pH 8.0 may be used in molecular biology as a washing buffer and potentially as an elution buffer for immuno micro sensors.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4080
BCBR2665V

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D A Baunoch et al.
BioTechniques, 12(3), 412-417 (1992-03-01)
In this paper we report on the evaluation of several procedures that allow for the repeated use of an antigen-coated, enzyme-linked immunosorbent assay (ELISA) plate for enzyme immunoassay (EIA). We have shown that antigen-coated ELISA plates that were incubated once
Tiegang Xu et al.
Biomedical microdevices, 14(2), 303-311 (2011-11-04)
With high sensitivity and specificity to antigen, immune micro-sensors can be used in rapid detection of pathogenic microbial. This study proposes and develops a method for rapidly regeneration of antibody on a resonant micro-cantilever sensor. A nitrilotriacetic acid (NTA) derivative

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