Fluorescence of an analyte, whether natural or induced by derivatization using chemicals such as 2-[N-(7-nitro-4-benzofurazanyl)methylamino]acethydrazide, can be leveraged to increase the sensitivity (detect lower levels) of the analysis. In addition, the uniqueness of fluorescent character can allow for the selective identification of a molecule in a complex mixture. Fluorescence is quantifiable at lower concentrations and usually has a wider linear range of response vs. concentration compared to optical (UV-VIS) absorbance.
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Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Derivatizing agent for fluorescence detection of carbonyl compounds with HPLC[1]
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Journal of pharmaceutical and biomedical analysis, 17(6-7), 1065-1070 (1999-01-12)
A new fluorescent reagent for carboxylic acids, N-(4-nitro-2,1,3-benzoxadiazoyl-7-yl)-N-methyl-2-aminoacetohydr azide (NBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography was examined. NBD-CO-Hz reacted with 2-arylpropionic acids (2-APAs), a group of non-steroidal antiinflammatory drugs (NSAIDs) in
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