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77339

Sigma-Aldrich

Pertussis toxin from Bordetella pertussis

Synonym(s):

Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen

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About This Item

CAS Number:
70323-44-3
MDL number:
MFCD00131841
UNSPSC Code:
12352200

storage temp.

−20°C

Gene Information

Bordetella pertussis Tohama I ... ptxA(2665068) , ptxB(2665069) , ptxC(2665408)

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Application

Inactive toxin form released from B. pertussis.
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.

Physical form

aseptically packaged solution in 50% (v/v) glycerol; 50 mM TRIS, pH 7.5; 10 mM glycine; 0.5 M NaCl

Other Notes

Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins; Pertussis toxin-catalyzed ADP-ribosylation of G proteins

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Skull and crossbones
GHS06

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins.
G Milligan
The Biochemical journal, 255(1), 1-13 (1988-10-01)
Pertussis toxin-catalyzed ADP-ribosylation of G proteins.
D J Carty
Methods in enzymology, 237, 63-70 (1994-01-01)
Michal Aharonowiz et al.
PloS one, 3(9), e3145-e3145 (2008-09-06)
Multiple sclerosis (MS) is an immune mediated demyelinating disease of the central nervous system (CNS). A potential new therapeutic approach for MS is cell transplantation which may promote remyelination and suppress the inflammatory process. We transplanted human embryonic stem cells

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