Product 75917 is an ingredient for BHI infusion broth. Typically, BHI broth acc. ISO 6888 contains Dehydrated Calf Brain Infusion 12.5 g/l, Dehydrated Beef Heart Infusion 5 g/l. Product 75917 is a porcine alternative to these ingredients and can be used at similar concentration, or following appropriate scientific literature.
75917
Brain Heart Infusion, Porcine
suitable for microbiology
Synonym(s):
BHI Broth, Brain Heart Broth
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500 G
$203.00
About This Item
UNSPSC Code:
41106212
NACRES:
NA.85
Recommended Products
biological source
Porcine heart
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
amino-nitrogen, ≥4.5%
total nitrogen (N), ≥11%
packaging
pkg of 500 g
storage condition
(Tightly closed. Dry.)
ign. residue
≤17%
loss
≤6.0% loss on drying
color
light yellow
pH
6.5-7.5 (25 °C, 2% in H2O)
solubility
H2O: 2%, clear, light yellow (and light brown-yellow and light brown)
application(s)
food and beverages
microbiology
General description
Brain Heart Infusion (BHI), Porcine, is a culture medium commonly used in microbiology for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures.BHI is typically prepared using porcine (pig) brain and heart tissues as the source of the raw materials. The enzymatic digestion of porcine brain-heart infusion provides amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.
Application
Brain Heart Infusion, porcine, is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium for the growth of Streptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes, and Clostridium difficile.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.
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How much powder should be used for 1000 ml?
1 answer-
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Can I have the composition of Brain Heart Infusion, Porcine ( Cat No. 75917). It requires a specific mass of each component in 1 liter of medium. Thank you very much.
1 answer-
While Brain Heart Infusion, catalog no. 75917, is typically prepared using porcine brain and heart tissues as the source of the raw materials, which are then enzymatically digested to yield amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms, the exact composition of this Brain Heart Infusion is proprietary and cannot be shared.
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