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57993

Sigma-Aldrich

Neutral Red solution

suitable for microscopy

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:

shelf life

limited shelf life, expiry date on the label

suitability

suitable for microscopy

SMILES string

[Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With basic dye, they tend to stain more strongly than the rest of the bacterium, and with methylene blue solution (Cat. No. 01363) they stain metachromatically, a reddish purple color.

Application

Neutral Red, a histological vital stain, is used as a stain and counterstain. It is used to stain Golgi apparatus in cells and Nissi granules in neurons. As a pH sensitive dye, neutral red stains lysosomes red within cells.

Components

Composition:
Neutral red 1.0 g/l, Acetic acid 2 ml/l in water

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Juan C Stockert et al.
Acta histochemica, 114(8), 785-796 (2012-02-22)
Although MTT is widely used to assess cytotoxicity and cell viability, the precise localization of its reduced formazan product is still unclear. In the present study the localization of MTT formazan was studied by direct microscopic observation of living HeLa
L Ivanova et al.
Chemical research in toxicology, 25(8), 1662-1674 (2012-06-27)
Enniatins are cyclic hexadepsipeptidic mycotoxins with ionophoric, antibiotic, and insecticidal activity. Enniatin B (EnnB), the most important analogue, is produced by many Fusarium species and is a common contaminant in grain-based foods. The compound's cytotoxic potential has been shown in
Cássio do Nascimento et al.
Microscopy research and technique, 76(2), 126-132 (2012-10-31)
The aim of this randomized crossover investigation was to assess the biofilm formation on two titanium and one zirconia substrates in relation to the topography and surface roughness of the materials. Twenty-four discs specimens for each evaluated material (Machined pure
Robim M Rodrigues et al.
Toxicology in vitro : an international journal published in association with BIBRA, 27(2), 760-767 (2012-12-25)
When in vitro test systems are evaluated for assessment of the toxicity of chemical compounds, particular efforts are made to mimic the in vivo reality as close as possible. Cellular models with appropriate metabolic competence, i.e. with the potency to
Kun Guo et al.
Biosensors & bioelectronics, 47, 184-189 (2013-04-13)
This study introduces a novel and simple method to covalently graft neutral red (NR) onto carbon surfaces based on spontaneous reduction of in situ generated NR diazonium salts. Immobilization of neutral red on carbon surface was achieved by immersing carbon

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