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51558

Sigma-Aldrich

HEPES buffered saline

BioUltra, Molecular Biology, 2× concentrate

Synonym(s):

HEPES Saline Buffer Solution

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50 ML
$110.00

$110.00

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50 ML
$110.00

About This Item

MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
329757705
NACRES:
NA.25

$110.00

Available to ship onMay 01, 2025Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES buffered saline is a tissue culture buffer,[1] which is used to maintain the pH of cell culture.[2] It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.[3]

Application

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA[4][5]
  • as a reagent to prepare transfection master mix for DNA construct vectors[6]
  • to develop hydrogel precursor for cell leakage assay[7]

Preparation Note

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH7391
BCCG6432
BCCD2118
BCBZ9638
BCBZ7440

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Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel
Hasselmann NF and Horn W
Biomedical Physics & Engineering Express, 4(3), 035004-035004 (2018)
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (2015)
In utero electroporation: Assay system for migration of cerebral cortical neurons
Electroporation and Sonoporation in Developmental Biology, 143-152 (2009)
Labeling embryonic mouse central nervous system cells by in utero electroporation
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Development, Growth & Differentiation, 50(6), 507-511 (2008)
C P Leamon et al.
Bioconjugate chemistry, 10(6), 947-957 (1999-11-24)
Poly(ethylene glycol) of various sizes was used as a molecular spacer to separate the cell-targeting ligand, folate, from the surface of poly-L-lysine. The resulting ternary macromolecule (pLys-PEG-folate) was investigated in various formulations for its ability to transfect reporter plasmids into
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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. whether the HEPES is dissolved in PBS? Additionally, how about the concentration of the HEPES in PBS?

    1 answer

    1. Per the components section on the Product Detail page, the composition of the product is as follows: concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose.

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