46345
Fiske-Subbarow reducer
Synonym(s):
Fiske-Subbarow reagent
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General description
formulation of 1-amino-2-naphthol-4-sulfonic acid, sodium sulfite and bisulfite according to C.H. Fiske and Y. Subbarow, J.Biol.Chem. 66, 375 (1925)
Application
The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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A Kyaw et al.
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
Eduardo H S Sousa et al.
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
E Durward et al.
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
The colorimetric determination of phosphorus
Fiske, C. H., Subbarow, Y.
The Journal of Biological Chemistry, 66, 375-375 (1925)
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