29222

Thio-H

• Synonym(s): 2-Amino-5-(5-phenyl-2-thienyl)phenol-N,N,O-triacetic acid tricesium salt
• Empirical Formula (Hill Notation): C₂₂H₁₆Cs₃NO₇S
• Molecular Weight: 837.15
• Beilstein/REAXYS Number: 9244017
• MDL number: MFCD03791074
• UNSPSC Code: 12352202
• PubChem Substance ID: 329753323

Properties

• form: solid
• fluorescence: λ_ex 332 nm; λ_em 500 nm in 0.1 M Tris pH 8.0, 10 mM Ca²⁺
• SMILES string: [Cs+].[Cs+].[Cs+].[O-]C(=O)COc1cc(ccc1N(CC([O-])=O)CC([O-])=O)-c2ccc(s2)-c3ccccc3
• InChI: 1S/C22H19NO7S.3Cs/c24-20(25)11-23(12-21(26)27)16-7-6-15(10-17(16)30-13-22(28)29)19-9-8-18(31-19)14-4-2-1-3-5-14;;;/h1-10H,11-13H2,(H,24,25)(H,26,27)(H,28,29);;;/q;3*+1/p-3
• InChI key: GBQDXALAYDCKJB-UHFFFAOYSA-K

Description

Features and Benefits

This is a new Calcium indicator with promising properties which provide an excellent addition to fluorescent Ca²⁺/Mg²⁺ indicators available already. It is a low-affinity Ca²⁺-indicators (K_d ca 55 μM). Such indicators are useful for determination of Ca²⁺ in intracellular compartments [or for monitoring steep increases in intracellular concentration of Ca²⁺ .
Compared to wellknown low-affinity Ca²⁺ indicators Thio-H is about three times brighter. This is based on strong molar absorption as well as high quantum yields. High fluorescent output allows low indicator concentrations to be used, which decreases the cation buffering effects of indicator. The indicator offers the interesting property of being both excitation and emission rationable, thus ratiomeric measurements are possible both in excitations as emission mode. Further spectroscopic data (0.1 M Tris, pH 8.0): low [Ca²⁺], Exc.max. 362 nm, Em.max. 500 nm; high [Ca²⁺], Exc.max. 332 nm, Em.max. 500 nm

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Intracellular, ratiomeric, fluorescent Ca²⁺/Mg²⁺ indicator with high brightness[1]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)