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12115

Sigma-Aldrich

Benzidine

purum p.a., ≥98.0% (N)

Synonym(s):

4,4′-Diaminobiphenyl

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About This Item

Empirical Formula (Hill Notation):
C12H12N2
CAS Number:
Molecular Weight:
184.24
Beilstein/REAXYS Number:
742770
EC Number:
MDL number:
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.25

grade

purum p.a.

Quality Level

assay

≥98.0% (N)

SMILES string

Nc1ccc(cc1)-c2ccc(N)cc2

InChI

1S/C12H12N2/c13-11-5-1-9(2-6-11)10-3-7-12(14)8-4-10/h1-8H,13-14H2

InChI key

HFACYLZERDEVSX-UHFFFAOYSA-N

Gene Information

human ... UGT1A4(54657)

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Application

Benzidine can be used to study bioactive small molecules and cell biology. Benzidine has been used to determine pollen viability and stigma receptivity of Angelica dahurica. Benzidine has also been used to study cross-linking of horseradish peroxidase adsorbed on polycationic films.
for spectrophotometric determination of CN-, NO2-, ClO3-, SO42-

Other Notes

Reagent for the det. of blood; Reagent for the det. of peroxidases.; Reagent for the spectrophotometric det. of CN-, NO2-, ClO3-, etc.
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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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J.L. Royer et al.
Analytical Letters, 6, 619-619 (1973)
THE BENZIDINE TEST: A CRITICAL REVIEW.
B J CULLIFORD et al.
Journal of forensic sciences, 9(1), 175-191 (1964-01-01)
E.A. Burns
Analytical Chemistry, 32, 1800-1800 (1960)
P D Josephy
Federation proceedings, 45(10), 2465-2470 (1986-09-01)
Benzidine oxidative activation may proceed by peroxidase-catalyzed one-electron oxidation via free radical intermediates, or by N-acetylation followed by monooxygenase-catalyzed N-hydroxylation. The peroxidase route has been examined by using horseradish peroxidase or prostaglandin H synthase in vitro. In the presence of
Brian J Pak et al.
PLoS neglected tropical diseases, 8(8), e3002-e3002 (2014-08-08)
Strongyloidiasis is a persistent human parasitic infection caused by the intestinal nematode, Strongyloides stercoralis. The parasite has a world-wide distribution, particularly in tropical and subtropical regions with poor sanitary conditions. Since individuals with strongyloidiasis are typically asymptomatic, the infection can

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