05-2605

CHAPS

≥97.0%

• Synonym(s): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₇S
• CAS Number: 75621-03-3
• Molecular Weight: 614.88
• MDL number: MFCD00012116
• UNSPSC Code: 12161900
• PubChem Substance ID: 329747670

Properties

• description: zwitterionic
• assay: ≥97.0%
• mol wt: micellar avg mol wt 6150
• availability: available only in Japan
• aggregation number: 10
• technique(s): dialysis: suitable; electrophoresis: suitable; protein quantification: suitable
• CMC: 6 mM (20-25°C)
• transition temp: cloud point >100 °C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Description

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Suitability

for biological purposes

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable