TAQNTDKB

KAPA Taq PCR Kit with dNTPs

Buffers with dye

• Synonym(s): PCR, dntp
• UNSPSC Code: 41106300
• NACRES: NA.56

Properties

• shelf life: ≤18 mo.
• feature: dNTPs included; hotstart: no
• packaging: pkg of 250 U (KK1021); pkg of 2500 U (BK1005); pkg of 500 U (KK1023); pkg of 5000 U (BK1007)
• manufacturer/tradename: Roche
• technique(s): PCR: suitable
• input: purified DNA
• storage temp.: −20°C

Description

General description

KAPA Taq is supplied in a 2X ReadyMix^™ format consisting all the components essential for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq DNA Polymerase is provided with two reaction buffers (Buffer A and Buffer B) or a single buffer with loading dye. It allows convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers, whereas KAPA Taq Buffer B is a Tris-potassium chloride buffer. KAPA Taq DNA Polymerase is used in combination with any standard Taq buffer with a pH of 8.3 or higher.

Application

KAPA Taq PCR Kit with dNTPs may be used for:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymerase chain reaction (PCR)[1]

Biochem/physiol Actions

KAPA Taq DNA Polymerase is a single-subunit, wild-type Taq DNA polymerase extracted from thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart^® DNA Polymerase shows 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The enzyme elicits error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In hot start formulation, the KAPA Taq combines with a proprietary antibody and inactivates the enzyme until the first denaturation step. It eliminates the spurious amplification of products and increases the reaction efficiency and sensitivity.

Features and Benefits

High performance:

• Improved sensitivity, specificity, and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

Quick Notes:

• KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
• The final MgCl₂ concentration may need to be optimized to account for differences in buffer formulation.
• KAPA Taq Buffers contain MgCl₂ at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed tocontain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endo nuclease activity, and meet strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• KAPA Taq DNA Polymerase 5 U/µL
• 10X KAPA Taq Buffer with loading dye
• MgCl₂ 25 mM
• KAPA dNTP Mix 10 mM each

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash