TAQNL-RO

Taq DNA Polymerase (1 U/μl), dNTPack

suitable for PCR, optimum pH ~9.0 (20 °C)

• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300

Properties

• usage: sufficient for ≤2,000 reactions (04738241001); sufficient for ≤500 reactions (04738225001)
• feature: dNTPs included; hotstart: no
• packaging: pkg of 1,000 U (04738241001 [4 x 250 U]); pkg of 250 U (04738225001)
• manufacturer/tradename: Roche
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• input: purified DNA
• optimum pH: ~9.0 (20 °C)
• storage temp.: −20°C

Description

General description

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.[1]

Application

Taq DNA Polymerase (1 U/μl), dNTPack may be used for:

• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 5 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Taq DNA Polymerase
• PCR Buffer with MgCl₂ 10x concentrated
• MgCl₂ Stock Solution
• PCR Buffer without MgCl₂
• PCR Nucleotide Mix</ul>

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash