11828681001
Roche
Annexin-V-FLUOS
solution, suitable for flow cytometry
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About This Item
UNSPSC Code:
12352200
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form
solution
usage
sufficient for 250 tests
packaging
pkg of 500 μL
manufacturer/tradename
Roche
technique(s)
flow cytometry: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Annexin-V-FLUOS is a phospholipid-binding protein with a high affinity for phosphatidylserine (PS). Detection of cell-surface PS with annexin-V thus serves as a marker for apoptotic cells. Analysis may be by flow cytometry or by fluorescence microscopy.
Specificity
Annexin-V binds in a Ca2+-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.
Application
In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner part of the plasma membrane to the outer layer, by which PS becomes exposed at the external surface of the cell.
Annexin-V-FLUOS detects apoptotic cells in suspension cells using flow cytometry (FACS)[1] or fluorescence microscopy, by binding to phosphatidylserine on the outer leaflet of apoptotic cell membranes.[2][3][4][5] A simultaneous labeling with, e.g., propidium iodide (PI) is needed for differentiation of apoptotic from necrotic cells.
Other secondary labeling is possible, e.g., membrane surface staining with a phycoerythrin or TRITC-labeled monoclonal antibody for further cellular characterization.
Annexin-V-FLUOS detects apoptotic cells in suspension cells using flow cytometry (FACS)[1] or fluorescence microscopy, by binding to phosphatidylserine on the outer leaflet of apoptotic cell membranes.[2][3][4][5] A simultaneous labeling with, e.g., propidium iodide (PI) is needed for differentiation of apoptotic from necrotic cells.
Other secondary labeling is possible, e.g., membrane surface staining with a phycoerythrin or TRITC-labeled monoclonal antibody for further cellular characterization.
Preparation Note
Working concentration: Amount of rGFP recommended for:
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
- SDS-PAGE (Coomassie stained): 1μg
- Western blot: 5ng
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
Other Notes
For life science research only. Not for use in diagnostic procedures.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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