04739205001
Roche
Biotin-11-CTP
solution, pkg of 25 μL (250 nmol; 10 mM)
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About This Item
UNSPSC Code:
12352200
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description
C28H44N7Na2O17P3S (Formula)
Quality Level
form
solution
mol wt
Mr 921.66 (Biotin-11-CTP-Na2)
packaging
pkg of 25 μL (250 nmol; 10 mM)
manufacturer/tradename
Roche
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
General description
Biotin is bound to cytidine triphosphate via an amide linkage.
Contents
Biotin-11-CTP, disodium salt, 10 mM solution
Contents
Biotin-11-CTP, disodium salt, 10 mM solution
Application
Biotin-11-CTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace CTP in the in vitro transcription reaction for RNA labeling.
Double-stranded DNA (i.e., linearized plasmid or cDNA) containing a T7, SP6, or T3 promoter serves as a template for in vitro transcription with the corresponding RNA polymerase, using ATP, GTP, CTP, UTP, and Biotin-11-CTP. For a higher and/or more uniform label density,
Biotin-11-CTP can be used together with Biotin-16-UTP in the in vitro transcription reaction.[1]
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Strepavidin-AP conjugate.
Double-stranded DNA (i.e., linearized plasmid or cDNA) containing a T7, SP6, or T3 promoter serves as a template for in vitro transcription with the corresponding RNA polymerase, using ATP, GTP, CTP, UTP, and Biotin-11-CTP. For a higher and/or more uniform label density,
Biotin-11-CTP can be used together with Biotin-16-UTP in the in vitro transcription reaction.[1]
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Strepavidin-AP conjugate.
Quality
Typical analysis: 85% Biotin-11-CTP (HPLC, area%)
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Stéphanie Anchisi et al.
mBio, 6(2), e02349-e02349 (2015-03-05)
Many RNA viruses are detected by retinoic acid-inducible gene i (RIG-I), a cytoplasmic sensor that triggers an antiviral response upon binding non-self-RNA that contains a stretch of double-stranded RNA (dsRNA) bearing a base-paired 5' ppp nucleotide. To gain insight into
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