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WBAVDP001

Millipore

Immobilon® Block - PO (Phosphoprotein Blocker)

Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

Quality Level

300

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent (phosphoprotein)
fluorometric (phosphoprotein)

shipped in

ambient

storage temp.

15-25°C

Quality

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Storage and Stability

Stable for 1 year at 20–25°C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Johnathan M Borland et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Like many social behaviors, aggression can be rewarding, leading to behavioral plasticity. One outcome of reward-induced aggression is the long-term increase in the speed in which future aggression-based encounters is initiated. This form of aggression impacts dendritic structure and excitatory
Vanesa D Ramseyer et al.
American journal of physiology. Renal physiology, 308(2), F149-F156 (2014-11-08)
Thick ascending limbs reabsorb 30% of the filtered NaCl load. Nitric oxide (NO) produced by NO synthase 3 (NOS3) inhibits NaCl transport by this segment. In contrast, chronic angiotensin II (ANG II) infusion increases net thick ascending limb transport. NOS3
H N Gao et al.
Journal of dairy science, 100(9), 7696-7709 (2017-06-26)
The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the
Riasat Zaman et al.
The Journal of cell biology, 220(6) (2021-04-10)
Activated ezrin-radixin-moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton to generate apical structures, including microvilli. Among many kinases implicated in ERM activation are the homologues LOK and SLK. CRISPR/Cas9 was used to knock out all ERM proteins
I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts
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Immunodetection

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

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