ST1711

Anti-DFHR Mouse mAb (2B10)

liquid, clone 2B10, Calbiochem^®

• Synonym(s): Anti-Dihydrofolate Reductase
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2B10, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: rat, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DHFR(1719)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.

Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.

This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

Immunogen

A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
HeLa cells, PC-12 Cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable