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ST1553

Sigma-Aldrich

Anti-VCP Mouse mAb (4A8)

liquid, clone 4A8, Calbiochem®

Synonym(s):

Anti-Valosin-Containing Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4A8, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCP(7415)

General description

Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.

Immunogen

Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HePG2 cells, human placenta tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Philipp Voisard et al.
International journal of molecular sciences, 23(12) (2022-06-25)
Valosin-containing protein (VCP) acts as a key regulator of cellular protein homeostasis by coordinating protein turnover and quality control. Mutations in VCP lead to (cardio-)myopathy and neurodegenerative diseases such as inclusion body myopathy with Paget's disease of the bone and

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