SCC050
PluriStem C57BL/6N Murine ES Cells
This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.
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About This Item
UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.81
Recommended Products
biological source
mouse
Quality Level
manufacturer/tradename
PluriSTEM®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
General description
Embryonic Stem Cell line derived from C57BL/6N blastocysts. C57BL/6N is the most commonly used inbred mouse strain. Large scale mouse knockout projects such as NIH’s KOMP project use C57BL/6 as the standard background for mouse genome engineering. C57BL/6 is also the reference strain for mouse genome sequencing at the NHGRI. C57BL/6N mice show good breeding performance. As a commonly used reference strain, transgenic mouse models are often backcrossed to a uniform C57BL/6 background, and B6 ES cells facilitate building complex models which combine gene inactivation with existing transgenics maintained in a B6 background. Additional phenotypic information is available at the Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C57BL.shtml). The B6 ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.
STRAIN: C57BL/6N
GENDER: Male
KARYOTYPE: 40XY
STRAIN: C57BL/6N
GENDER: Male
KARYOTYPE: 40XY
Cell Line Description
Embryonic Stem Cells
Application
Research Category
Stem Cell Research
Stem Cell Research
This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.
Packaging
2.5 x 106 cells per vial, passage 9
Physical form
Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO
Storage and Stability
Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.
Legal Information
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Cameron Moshfegh et al.
Stem cell research, 61, 102780-102780 (2022-04-09)
Spermatogonial stem cells (SSCs) originate from gonocytes that differentiate from primordial germ cells (PGCs). In the developing mouse testis, expression of the gene LIM homeobox 1 (Lhx1) marks the most undifferentiated SSCs, which has not yet been reported for spermatogonia-like
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